antibodies against nrf2  (Cell Signaling Technology Inc)

Journal: Journal of dairy science

Article Title: Cullin3 mitigates NEFA-induced oxidative stress in mammary epithelial cells: involvement of BCL2/BECN1 and autophagy.

doi: 10.3168/jds.2024-25879

Figure Lengend Snippet: Figure 6. CUL3 does not exacerbate NEFA-induced reductions in mRNA levels of NFE2L2 downstream target genes. A–B Western blot analysis of NFE2L2. C qRT-PCR analysis of relative mRNA levels of NFE2L2. D Immunofluorescence analysis of NFE2L2 (red) and nuclear staining with DAPI (blue), scale bar = 25 μm. E-H qRT-PCR analysis of relative mRNA levels of GCLC (E), GCLM (F), HMOX1 (G) and NQO1 (H). One-way ANOVA was used for significant difference analysis. Data are presented as the mean ± SEM. Different superscript lowercase letters in bar charts represent significant difference (P < 0.05)

Article Snippet: MAC-T cells were then incubated overnight at 4°C with a primary antibody against NFE2L2 (1:400, 12721, CST), followed by a 1 h incubation at 37°C with a TRITC-conjugated secondary antibody (1:200, AS040, ABclonal).

Techniques: Western Blot, Quantitative RT-PCR, Immunofluorescence, Staining

Journal: Frontiers in Cell and Developmental Biology

Article Title: Formononetin promotes porcine oocytes maturation and improves embryonic development by reducing oxidative stress

doi: 10.3389/fcell.2024.1520429

Figure Lengend Snippet: Information about the primer sequences.

Article Snippet: And incubation with horseradish peroxidase-conjugated secondary antibodies at RT for 1 h. Primary antibodies were rabbit monoclonal antibodies against NRF2 (1:2000, #16396-1-AP, Proteintech), KEAP1 (1:2000, #10503-2-AP, Proteintech), and β-Actin (1:5000, #20536-1-AP, Proteintech).

Techniques:

Journal: Frontiers in Cell and Developmental Biology

Article Title: Formononetin promotes porcine oocytes maturation and improves embryonic development by reducing oxidative stress

doi: 10.3389/fcell.2024.1520429

Figure Lengend Snippet: Effect of FMN on mitochondrial function in oocytes and early embryos. (A) The mitochondria distribution at MII stage were stained with Mito-Tracker Red; scale bar, 50 μm. (B) The relative abundance of mitochondria in oocyte were analyzed for control and FMN-treated group. (C) Relative mRNA levels of ATP5B, NRF2 and KEAP1 in oocytes. (D) 4-cell stage embryos stained with JC-1. Scale bar, 100 μm. (E) Relative levels of JC-1 Red/Green fluorescence intensity in embryos were analyzed for control and FMN-treated group. (F) Relative mRNA levels of TFAM and NRF1 in embryos. (G) ATP levels of 4-cell stage embryos in both control and FMN-treated groups. Data are presented as the mean ± standard deviation (SD). * p < 0.05,** p < 0.01, *** p < 0.001 vs. 0 μM FMN group.

Article Snippet: And incubation with horseradish peroxidase-conjugated secondary antibodies at RT for 1 h. Primary antibodies were rabbit monoclonal antibodies against NRF2 (1:2000, #16396-1-AP, Proteintech), KEAP1 (1:2000, #10503-2-AP, Proteintech), and β-Actin (1:5000, #20536-1-AP, Proteintech).

Techniques: Staining, Control, Fluorescence, Standard Deviation

Journal: Frontiers in Cell and Developmental Biology

Article Title: Formononetin promotes porcine oocytes maturation and improves embryonic development by reducing oxidative stress

doi: 10.3389/fcell.2024.1520429

Figure Lengend Snippet: Effect of FMN on the expression levels of the Nrf2/Keap1 signaling pathway-related protein and genes in porcine blastocysts. (A) Porcine blastocysts incubated with or without FMN were stained with NRF2 (green) and DAPI (blue), scale bar, 50 μm. (B) Relative levels of NRF2 fluorescence intensity in blastocysts were analyzed for the control and FMN-treated group. (C) Relative mRNA expression levels of genes related to Nrf2/Keap1 pathway, NRF2, KEAP1, NQO1, UCHL1 in blastocysts. (D) Western blot analysis of NRF2 and KEAP1 protein expressions in blastocysts in the control and FMN-treated groups. Data were presented as the mean ± standard deviation (SD). ** p < 0.01,*** p < 0.001 vs. 0 μM FMN group.

Article Snippet: And incubation with horseradish peroxidase-conjugated secondary antibodies at RT for 1 h. Primary antibodies were rabbit monoclonal antibodies against NRF2 (1:2000, #16396-1-AP, Proteintech), KEAP1 (1:2000, #10503-2-AP, Proteintech), and β-Actin (1:5000, #20536-1-AP, Proteintech).

Techniques: Expressing, Incubation, Staining, Fluorescence, Control, Western Blot, Standard Deviation

Journal: Frontiers in Cell and Developmental Biology

Article Title: Formononetin promotes porcine oocytes maturation and improves embryonic development by reducing oxidative stress

doi: 10.3389/fcell.2024.1520429

Figure Lengend Snippet: Summary of the effect of Formononetin (FMN) on porcine oocyte in vitro maturation and early embryo in vitro . This schematic view illustrates that during the IVM stage, FMN enhances oocyte maturation in vitro by up-regulating genes related to CCs expansion and antioxidants, reducing oxidative stress, and improving mitochondrial function. During the IVC stage, FMN promotes the nuclear expression of Nrf2 through the P62-Keap1-Nrf2 pathway, which exerts antioxidant function and enhances mitochondrial function. Then, FMN promotes cell proliferation, reduces apoptosis, and lowers autophagy levels.

Article Snippet: And incubation with horseradish peroxidase-conjugated secondary antibodies at RT for 1 h. Primary antibodies were rabbit monoclonal antibodies against NRF2 (1:2000, #16396-1-AP, Proteintech), KEAP1 (1:2000, #10503-2-AP, Proteintech), and β-Actin (1:5000, #20536-1-AP, Proteintech).

Techniques: In Vitro, Expressing